Objectives: This study aimed to confirm the degree of gender sensitivity, gender role conflict, nursing professionalism of nursing students and the factors that affect nursing professionalism. Methods: During Jan. 19 to Feb. 5 in 2021, the structured questionnaire was used for 187 nursing students by on-line research methods. Data were analyzed by descriptive analysis, mean comparison(t-test, ANOVA), correlation analysis(Pearson's correlation coefficient) and multiple regression using SPSS/WIN 25.0. Results: The gender sensitivity had positive relationship with nursing professionalism, and gender role conflict had negative relationship with nursing professionalism. And the prediction factors influencing nursing professionalism were major satisfaction, gender sensitivity and gender role conflict. The total variance was 8.2% by predictors. Conclusions: In order to improve the nursing professionalism of nursing students, various ways to increase the satisfaction level of major should be sought, and program should be prepared to improve gender sensitivity and reduce gender role conflict.