Objective: The purpose of this study is to examine the Korean women’s attitude toward sex in their 20s in order to investigate the relationship between attitude toward sex and preventive behavior against cervical cancer. Method: The data was collected by conducting a survey in parts of Chungcheongbuk provinces from November 1st to the 10th, 2017, with a total of 353 respondents. Frequency analysis, chi-square test and logistic-regression analysis were conducted. Result: A result of logistic-regression analysis, the degree of preventive behavior against cervical cancer of those who were ‘conservative’ toward sex was 1.866 times greater, significantly. As the attitude toward sex becomes more liberal, the awareness of the necessity for preventive behavior against cervical cancer may possibly become lower. Conclusion: Therefore it is necessary to work in tandem with the school health program to increase preventive behavior against cervical cancer for the women in their 20s through early education and promotion.